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Can I run a melt curve on the Franklin Thermocycler?

Absolutely! The entire Biomeme platform is melt curve capable. 

Creating and running a Heating or Cooling Melt Curve protocol for your Franklin Thermocycler on the Biomeme Go mobile app is a simple process. 

Please follow the steps below to create your own Heating Melt Curve protocol:

  1. Log in to your Biomeme Go mobile app and tap "Protocol Management"
  2. Tap "+Add"
  3. Tap to input your desired protocol name 
  4. Toggle on the "Heating Melt" option 
  5. Set your desired parameters:
    • Melt Starting Temperature (C°) Minimum starting temperature is 35 
    • Melt Ending Temperature (C°) Ending temperature must be greater than the starting temperature
    • Melt Increment (C°) Options include: 0.5 or 1 
  6. Tap "Confirm"
  7. Return to your Home Screen
  8. Tap "Start Run" and select your newly created Heating Melt Curve protocol

Please follow the steps below to create your own Cooling Melt Curve protocol:

1. Log in to your Biomeme Go mobile app and tap "Protocol Management"

2. Tap "+Add"

3. Tap to input your desired protocol name

4. Toggle on the "Cooling Melt" option 

5. Set your desired parameters:

  • Melt Starting Temperature (C°) Starting temperature must be less than the ending temperature
  • Melt Ending Temperature (C°) Minimum ending temperature is 35C° 
  • Melt Increment (C°) Options include: 0.5 or 1 

6. Tap "Confirm"

7. Return to your Home Screen

8. Tap "Start Run" and select your newly created Cooling Melt Curve protocol


Important Note:

PCR and Melt Curve are separate runs on our system. After your PCR finishes, create a new Melt Curve run (following the steps above). The PCR and Melt Curve data will be stored as separate runs within the app and on Biomeme Cloud.

 


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